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Objective To investigate the clinical features and survival status of mucosal melanoma of the nasal cavity and paranasal sinuses and to analyze the prognostic factors.Methods A retrospective analysis was performed on the clinical data of 94 patients with mucosal melanoma of the nasal cavity and paranasal sinuses treated from January 2000 to December 2012.Of the 94 patients,50 were male,and 44 were female.The median age of onset was 60 years (range,26-85 years).The primary sites were nasal cavity (86 patients),maxillary sinus (7 patients),and nasopharynx (1 patient).Cervical lymph node metastasis was observed in 10 patients (7 patients before treatment,2 patients during treatment,and 1 patient after treatment).No patient had distant metastasis.Patients were treated with surgery ± radiotherapy.The Kaplan-Meier method was used to calculate survival rates,and the logrank test was used for univariate prognostic analysis;the Cox regression model was used for multivariate prognostic analysis.Results The 1-,3-,and 5-year sample sizes were 80,54,and 50,respectively.The 1-,3-,and 5-year disease-related survival rates were 71％,33％,and 22％,respectively.Univariate analysis showed that the prognostic factors were age over 55 years (P =0.034),involvement of the posterior naris (P =0.011),involvement of the maxillary sinus (P =0.009),involvement of the hard palate (P =0.003),cervical lymph node metastasis (P =0.001),and therapeutic method (P =0.038).Multivariate analysis showed that the prognostic factors were involvement of the posterior naris (P =0.027),involvement of the orbit (P =0.005),and involvement of the hard palate (P =0.003).Conclusions The distant metastasis and local recurrence rates are high among patients with mucosal melanoma of the nasal cavity and paranasal sinuses,so combination therapy is imperative.Cervical lymph node metastasis rate is low.Rational clinical staging needs to be further explored.
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Objective To investigate the protective effects of reducing average radiation dose and increasing protective weight on the auditory system (tympanic cavity,the bony portion of eustachian tube,vestibule,and cochlea) during intensity-modulated radiotherapy (IMRT) for nasopharyngeal carcinoma (NPC).Methods The planning system (ADAC Pinnacle3 8.0m) with direct machine parameter optimization was used to optimize the IMRT planning for 40 patients with NPC (stage Ⅰ + Ⅱ:20 patients ;stage Ⅲ + Ⅳ:20 patients).Without reducing the radiation dose for target volume,the IMRT planning was optimized by limiting the average dose administered to the auditory system or increasing the protective weight for the protected organs in auditory system.The protective effects were assessed by analyzing the average dose received by the auditory system.Results After limiting the average dose administered to the auditory system without reducing the radiation dose for target volume,the average dose received by the auditory system was significantly reduced (3855.5-5391.3 Gy vs 2960.3-4559.6 Gy,P =0.000 for all) ; when the protective weight for the auditory system was increased,the average dose received by the auditory system was even more reduced (3855.5-5391.3 Gy vs 2725.4-4271.4 Gy,P =0.000 for all).For all three regimens,the average dose was significantly higher in stage Ⅲ + Ⅳ patients than in stage Ⅰ + Ⅱ patients (P =0.000 for all).Conclusions For the IMRT planning for NPC,limiting the average dose administered to the auditory system can greatly reduce the average dose received by the auditory system,and increasing the protective weight for the auditory system can further reduce the average dose received by the auditory system.However,the protective effect on the auditory system may be reduced as the stage of NPC increases.
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OBJECTIVE To establish a rat model of pulmonary infection by inoculating Pseudomonas aeruginosa to Sprague-Dawley(SD) rats and evaluate it.METHODS Two hundred SD rats were divided into 2 groups: the P.aeruginosa group and the control group,P.aeruginosa was embedded in minute seaweed alginate beads by an ejection set with an acuminate hole.Then the beads were inoculated into the rats′ lung through tracheal intubation.RESULTS The bacteriological values: P.aeruginosa was detected from rats of infected groups.Bacterial number was higher than 105CFU/g 3 and 7 days after infection and higher than 103CFU/g 14 and 28 days after infection.The pathological changes showed: 3 and 7 days after infection,lung abscess,edema,and consolidation could be seen from lungs of infected groups.At optical microscopy,alginateP.aeruginosa caused a pronounced inflammatory reaction with polymorphonuclear cells surrounding a bead.Fourteen and 28 days after infection,fibrinous adhesions and granulomas became the major pathological changes.CONCLUSIONS The animal model of pulmonary infection can be established by inoculating P.aeruginosa embedded in minute seaweed alginate beads made by an ejection set with an acuminate hole to SD rats.
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Glechoma hederocea agglutinin (Gleheda) is a novel glycosylated lectin isolated from the leaves of G. hederacea. Like other glycosylated proteins, the detection of Gleheda by immunological methods is often hampered by the cross-reactivity of the polyclonal antibodies with unrelated glycoproteins. Hence a protocol to purify monospecific polyclonal antibodies from a crude antiserum raised against Gleheda was developed. After selective ammonium sulfate precipitation and successive affinity chromatography on columns of Sepharose 4B with immobilized Gleheda and Robinia pseudoacacia agglutinin (RPA), respectively, ion-exchange chromatography on a column of Q Fast Flow was used for further purification. The specificity of the antibody fractions from each step was tested by double immunodiffusion assay and analyzed by Western blot. Results revealed that affinity chromatography of the immunoglobulin fraction on the immobilized Gleheda antigen yielded an antibody preparation that still cross-reacted with many proteins in leaf extracts. Depletion of nonspecific cross-reacting antibodies directed against the glycan part of the glycoprotein by affinity chromatography on immobilized RPA removed most but not all nonspecifically reacting antibodies. Only upon further purification by ion exchange chromatography an IgG fraction of monospecific antibodies that reacted exclusively with Gleheda could be obtained and accordingly was suitable for immunodetection studies. This antibody purification procedure promises simplicity and efficiency. In addition, this method does not require expensive facilities.
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Objective To establish a rat model of chronic pulmonary infection by inoculating Pseudomonas aeruginosa to Sprague-Dawley (SD) rats, and to evaluate the animal model with bacteriological and pathological stndies. Methods 240 healthy, clean SD rats were randomly divided into 2 groups: the infected group and the control group. Pseudomonas aeruginosa were embedded in minute seaweed alginate beads using an ejection set with an acuminate hole. Then the beads were inoculated into the rats′ lung through tracheal intubation. The bacteria number in the lung and pathological scores were determined 3, 7, 14 and 35 days after inoculation. The control group was treated with the same method using the sterile normal saline instead of the bacteria suspension. Results Bacteriological values: no bacterium was detected in the control group. Pseudomonas aeruginosa was detected from the rats of infected group in which the bacterial number was up over 105cfu/g at 3 and 7 days after infection, and up over 103cfu/g at 14 and 35 days after infection. Pathological changes: at 3 and 7 days after infection, lung edema, consolidation and hemorrhage could be seen in rat lungs of the infected group. Under the optical microscope, alginate-Pseudomonas aeruginosa caused a pronounced inflammatory reaction with polymorphonuclear cells surrounding beads, and microcolonies formed at the periphery of beads were also seen. Atelectasis and fibrosis and granalation were the major pathological changes at 14 and 35 days after infection. While in the control group, there were little changes after the inoculation except mild congestion and inflammatory reaction in the lungs 3 days after inoculation. Conclusion The rat model of chronic pulmonary infection can be reproduced by inoculating Pseudomonas aeruginosa embedded in minute seaweed alginate beads.